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KMID : 0364819910290040221
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Korean Journal of Microbiology
1991 Volume.29 No. 4 p.221 ~ p.225
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Analysis of RNA Transcripts Generated by Bluetongue Virus Core
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Yang, Jai Myung/양재명
Manning, JaRue S./Manning, JaRue S.
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Abstract
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1
The RNA transcripts produced from in vitro transcription reaction of BIN core were analyzed on agarose-urea gel. Fast migrating abortive RNAs, in addition to full length species of RNA, were observed. Fast migrating RNAs extracted from agarose-urea gel were hybridized to all 10 segments of genomic ds RNA, while slow migrating RNAs extracted from agarose-urea gel were hybridized only to the large and medium size genomic ds RNA. These results indicate that fast migrating RNA transcripts are most likely the products of abortive transcription.
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KEYWORD
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